Production of isotope-labeled proteins in insect cells for NMR
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چکیده
منابع مشابه
Production of Recombinant Proteins in Insect Cells
Among the wide range of methods and expression hosts available for the heterologous production of recombinant proteins, insect cells are ideal for the production of complex proteins requiring extensive posttranslational modification. This review article provides an overview of the available insect-cell expression systems and their properties, focusing on the widely-used Baculovirus Expression V...
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15 صفحه اولCell-free synthesis of 15N-labeled proteins for NMR studies.
Modern cell-free in vitro protein synthesis systems present powerful tools for the synthesis of isotope-labeled proteins in high yields. The production of selectively 15 N-labeled proteins from 15 N-labeled amino acids is particularly economic and yields are often sufficient to analyze the proteins very quickly by two-dimensional NMR spectra recorded of the crude reaction mixture without concen...
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ژورنال
عنوان ژورنال: Journal of Biomolecular NMR
سال: 2018
ISSN: 0925-2738,1573-5001
DOI: 10.1007/s10858-018-0172-7